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WebbHIS2作为报告基因时，会有一定程度的泄漏性表达，出现背景过高的现象，一般称作自激活。这时可 以在筛选培养基中加入适量的HIS2竞争性抑制剂3-AT（3-amino-1,2,4-triazole），以降低背景；或者增加 一个筛选标记Ade来进行更加严格的筛选。
His2.cat.com login
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WebbA donor construct and a gene knockout method, as well as a system and kit for the gene knockout are provided. The donor construct is a linear donor DNA or can be cleaved in a cell to produce the linear donor DNA. The gene knockout method uses a marker gene contained in the donor construct to enrich cells in which a gene is knocked out, thereby … WebbLogin using two factor authentication Powered by DHIS 2 [ Change language ] Arabic Arabic (Egypt) Arabic (Iraq) Arabic (Sudan) Bangla Bislama Burmese Central Kurdish Chinese Chinese (China) Czech Danish Dutch English French Indonesian Indonesian (Indonesia) Khmer Kinyarwanda Lao Mongolian Nepali Norwegian Bokmål Pashto …
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WebbThermal cycling was conducted in a Bio-Rad T100 (Cat. No. 1861096) under the following conditions: 95 C for 5 min, 34 cycles of 95 C for 30 s, 58.7 C for 30 s, 72 C for 60 s and final extension of 10 min at 72 C. Amplicons of expected size (690 bp) were cloned into pGEM-T Easy Vector (Promega, Cat. No. A1360) and Sanger sequenced at the …
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