Ephrina1-fc

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August undefined, 2024

WebJessica L Dries's 5 research works with 90 citations and 157 reads, including: Intramyocardial administration of chimeric ephrinA1‐Fc promotes tissue salvage following myocardial infarction in mice WebAug 13, 2014 · We have previously shown that EphrinA1/EphA expression profile changes in response to myocardial infarction (MI), exogenous EphrinA1-Fc administration following MI positively influences wound healing, and that deletion of the EphA2 Receptor (EphA2-R) exacerbates injury and remodeling. To determine whether or not ephrinA1-Fc would be …

Intracardiac administration of ephrinA1-Fc preserves …

WebDec 15, 2024 · EphrinA1-Fc administration in I/R preserves mitochondrial respiratory control under physiologically-relevant thermodynamic constraints To gather further … WebMar 21, 2009 · (C) HeLa cells transfected with the indicated plasmids were stimulated with pre-clustered ephrinA1-Fc (2 μg/ml) in serum-free medium for 5 min. GTP-bound Rac1 was identified by SDS–PAGE and immunoblotting, and its levels were measured and normalized to the corresponding total Rac1 levels. omfg food truck

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WebMay 10, 2024 · Furthermore, treatment of EphA2-KO cells with ephrinA1-Fc did not significantly decrease the rate of migration . EphrinA1-Fc is a promiscuous ligand and could in principle activate several EphA or EphB receptor subtypes , hence any inhibitory effects observed when treating cells with ephrinA1-Fc may be due to a variety of Eph subtypes. WebApr 14, 2024 · Akt inhibition and ephrinA1-Fc were used to confirm the influence of Akt activation and ephrinA1 levels on the EphA2 effects. Immunohistochemistry (IHC) was performed on xenograft and patient melanoma tissues. Results We found that high levels of ephrinA1, but not EphA2, were negatively correlated with melanoma metastasis. WebMar 30, 2011 · Biotinylated ephrinA1-Fc (R&D BT602) was added at 37°C for 4 hours at 30 ng/ml in displacement assays or in a range from 1 to 2000 ng/ml in saturation studies. omfg - hello lyrics

EphA2 Receptor and EphrinA1 Ligand in Solid Tumors: Function …

WebAug 21, 2015 · EphrinA1 induced a transient translocation of EphA2-mCitrine to the PM (Fig. 5a) followed by its clearance and subsequent appearance in Rab5-positive early endosomes45 (Supplementary Movie 1). Immunofluorescence of endogenous EphA2 and Rab5 confirmed this extensive colocalization after ephrinA1-Fc stimulation … Webmigrated at the level similar to EphrinA1-Fc purified from 293 cells stably expressing EphrinA1-Fc. No specific bands were detected in cell lysates or supernatants of 293 or MDBK mock-infected or infected with HAd-EphrinA1-Sc or HAd-ΔE1E3. These results suggest that HAd-EphrinA1-Fc can induce efficient expression and secretion of … omfg hello sheet musicWebSep 20, 2013 · EphrinA1/Fc is a soluble recombinant ephrinA1 fused to the Fc portion of human IgG. Our laboratory and others have previously shown that ephrin mimetic peptides induce receptor phosphorylation, degradation and downstream signaling as efficiently as ephrinA1 on the surface of ligand-expressing cells through juxtacrine contact 9, 22. omfg mashed potatoes lyrics

"WebDec 1, 2008 · EphrinA1 is a 205-amino acid, tumor necrosis factor-α early-inducible gene product located within chromosomal region 1q21-q22 and having a predicted molecular mass of 22 kDa. " - Ephrina1-fc

Ephrina1-fc

EphA2 Signaling Following Endocytosis: Role of Tiam1

WebApr 23, 2024 · While treatment with ephrinA1 (Fc- and His-tagged) increased the tumor-suppressive EphA2-pY588 in these cells, as expected upon ligand-mediated receptor … WebEphrin-A1, also known as B61 and LERK-1, is a member of the Ephrin-A family of GPI-anchored ligands that bind and induce the tyrosine autophosphorylation of Eph …

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WebNov 22, 2011 · EphA10优先与 EphrinA 结合， EphrinA5结合优先于与 EphrinA1 EphrinA2的结合，E- phA10 也能够与EphrinB1 和EphrinB2 弱结合，系统发育比较及配体结合显示这种弱结合与 EphA7 Eph家族蛋白的表达特点大多数Eph 受体成员主要在神经系统表达，但各自的表达有所区别又有所重叠脏 ... WebNov 1, 2002 · Recombinant EphA2-Fc and ephrinA1-Fc proteins were either purified from culture supernatant of stable 293T clones expressing these factors using protein A-Sepharose column, or purchased from R&D Systems, Inc. (Minneapolis, MN). The rabbit polyclonal antibody against the nonconserved spacer region of ephrinA1 was provided …

WebInfection with HAd-EphrinA1-Fc (HAd vector expressing extracellular domain of human EphrinA1 attached to Fc portion of human IgC 1 heavy chain) caused increased EphA2 activation and turnover and consequently decreased tumor cell viability in soft agar assays. Consistent with this observation, infection of MDA-MB-231 cells with HAd-EphrinA1-Fc ... WebApr 23, 2024 · While treatment with ephrinA1 (Fc- and His-tagged) increased the tumor-suppressive EphA2-pY588 in these cells, as expected upon ligand-mediated receptor activation, this phosphorylation was not...

WebApr 26, 2024 · EphA2 is a member of the receptor tyrosine kinase (RTK) Eph family and has many important and diverse biological functions, such as roles in tumor angiogenesis, … WebAims: Intracardiac injection of recombinant EphrinA1-Fc immediately following coronary artery ligation in mice reduces infarct size in both reperfused and non-reperfused myocardium, but the ...

WebEphrinA1; Ephrin-A1; EPLG1TNF alpha-induced protein 4; Immediate early response protein B61; LERK1; LERK1LERK-1; ligand of eph-related kinase 1; TNFAIP4B61; …

WebActive Recombinant human Ephrin A1 protein (Fc Chimera) is a HEK 293 Full length protein 19 to 182 aa range, > 95% purity, < 1.000 Eu/µg endotoxin level and validated in FuncS, SDS-PAGE. Measured by … omf fracture omfg mashed potatoesWebDec 15, 2024 · Collectively, these data suggest that intracardiac injection of EphrinA1-Fc protects cardiac function by preserving cardiomyocyte structure and mitochondrial … omfg i love you sheet musicWebThe Expression of ephrinA1/ephA2 Receptor Increases in Chronic Rhinosinusitis and ephrinA1/ephA2 Signaling Affects Rhinovirus-Induced Innate Immunity in Human … omfg hello piano 1 hourWebMar 22, 2024 · Also, EA1-Fc injection reduced protein levels of nuclear factor κ light-chain enhancer of activated B cells (NF-κ B) . NF-κ B is known to be involved with mediating inflammation . Taken together, these studies demonstrate robust multitarget properties of ephrinA1-Fc that coordinately reduce injury and promote tissue survival. omfg ice cream 10 hoursWebJul 7, 2024 · EphrinA1 and ephA2 membrane receptors may mediate “repulsive interaction” between adjacent cells, which belong to the largest receptor tyrosine kinase family.12In … omfg meant for you lyricsWebOct 1, 2024 · To determine if a correlation exists in this case, we fit a linear function to the data points for m-ephrinA1 and the peptide ligands, again excluding ephrinA1-Fc. Comparing the slope to the null hypothesis of 0 slope yielded a p value <0.01, which is indicative of a significant correlation ( Table S1 ). omfg music festival